Immunohistochemistry Kits and Reagents
Cayman Chemical HDAC Fluorometric Activity Assay Kit, 96 wells
HDACs catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcriptional activation to ensue. Transcriptional repression of key genes has linked HDAC activity to cell growth and tumor development. Cayman’s HDAC Activity Assay provides a fast, fluorescence-based method for measuring Class I and II HDAC activity that eliminates radioactivity, extraction, or chromatography. The procedure requires only two easy steps, both performed in the same microplate. The fluorescent reaction product is analyzed using a plate reader with excitation wavelengths between 340-360 nm and emission wavelengths between 440-465 nm.
Seracare Life Sciences Inc BIOTIN-SA HRP KIT MS PRI AB
HistoMark Biotin Streptavidin-HRP Systems provide rapid, precise localization of cell surface and intracellular antigens in frozen or paraffin-embedded tissue, cytospins and touch preparations. These systems also facilitate double or triple labeleing experiments, as detection can be performed simultaneously with primary antibodies of different animal species Streptavidin demonstrates less non-specific binding than avidin. Biotinylated anti-mouse antibody is human serum adsorbed to minimize cross-reactivity.
Cayman Chemical Glutathione Assay Kit, 96 wells
Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol. The GSH Assay can be used for plasma, tissue samples, and cultured cells with minimal sample processing.
Cayman Chemical TBARS (TCA Method) Assay Kit, 96 wells
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such as malondialdehyde (MDA). MDA can be quantified through a controlled reaction with thiobarbituric acid, generating 'Thiobarbituric Acid Reactive Substances' (TBARS). Cayman’s TBARS (TCA Method) Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates. The MDA-TBA adduct formed by the reaction of MDA and TBA under high temperature (90-100°C) and acidic conditions can be measured either colorimetrically at 530-540 nm or with much higher sensitivity fluorometrically at an excitation wavelength of 530 nm and an emission wavelength of 550 nm.
CELL MARQUE CORPORATION TRILOGY 1L
Trilogy (with EDTA), 1L, Applications: Immunohistochemistry, Stable indefinitely at room temperature, Biodegradable and Non-Toxic.
Biogenex Laboratories Super SensitiveTM (SS) Link-Label IHC Detection System
A classic system based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound antibody. These kits include multi-Link – a mix of anti-mouse and anti-rabbit IgGs conjugated to multiple biotin molecules and a Label -Strepavidin conjugated with an enzyme (horseradish peroxidase (HRP) or alkaline phosphatase (AP))
MilliporeSigma T DIAGNOSTICS153 PARAINFLUE
Life Sciences,Clinical Diagnostics & Infectious Disease,LIGHT DIAGNOSTICS Parainfluenza 1 Antibody FITC Reagent; ~50 tests; included in kit #3110; #3120; & #3137
Cayman Chemical MitoCheck Complex I Activity Assay Kit, 96 wells
Complex I (NADH oxidase/Co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I.
MilliporeSigma T DIAGNOSTICS153 SIMULFLUOR
SimulFluor HSV1/2 Reagent - One 5 mL dropper vial containing two components specific for HSV-1 and HSV-2, protein stabilizer, Evans blue and 0.1% sodium azide (preservative).
Cayman Chemical FTY720 Phosphate, 402615-91-2, 5 mg
Molecular Formula C19H34NO5P, Purity ≥98%, Formula Weight 387.5, Synonyms: FTY720P; A potent agonist at four of the sphingosine-1-phosphate (S1P) receptors with IC50 values of 0.2-6 nM.